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il 6 elisa kit  (R&D Systems)


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    R&D Systems il 6 elisa kit
    (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐originated lymph vessels. (b) Effects of water intake on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake on changes in the concentration <t>of</t> <t>IL‐6</t> in the lymph. (d) Effects of water intake on changes in the concentration of IL‐10 in the lymph. The open column, control; the black column, water intake. The error bars represent SDs.
    Il 6 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+il+6/Rat+IL-6+Quantikine+ELISA+Kit/pmc13128524-47-30-36
    Average 96 stars, based on 1273 article reviews
    il 6 elisa kit - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10"

    Article Title: Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10

    Journal: Physiological Reports

    doi: 10.14814/phy2.70891

    (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐originated lymph vessels. (b) Effects of water intake on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake on changes in the concentration of IL‐6 in the lymph. (d) Effects of water intake on changes in the concentration of IL‐10 in the lymph. The open column, control; the black column, water intake. The error bars represent SDs.
    Figure Legend Snippet: (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐originated lymph vessels. (b) Effects of water intake on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake on changes in the concentration of IL‐6 in the lymph. (d) Effects of water intake on changes in the concentration of IL‐10 in the lymph. The open column, control; the black column, water intake. The error bars represent SDs.

    Techniques Used: Concentration Assay, Control

    (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐derived lymph vessels in the absence (white column) and presence of clodronate (oblique line column). (b) Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐1β in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐6 in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐10 in the lymph. The error bars represent SDs.
    Figure Legend Snippet: (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐derived lymph vessels in the absence (white column) and presence of clodronate (oblique line column). (b) Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐1β in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐6 in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐10 in the lymph. The error bars represent SDs.

    Techniques Used: Derivative Assay, Concentration Assay

    (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐derived lymph vessels in the absence (white column) and presence of a MyD88 inhibitor (oblique line column). (b) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐6 in the lymph. (d) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐10 in the lymph. The error bars represent the SDs.
    Figure Legend Snippet: (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐derived lymph vessels in the absence (white column) and presence of a MyD88 inhibitor (oblique line column). (b) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐6 in the lymph. (d) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐10 in the lymph. The error bars represent the SDs.

    Techniques Used: Derivative Assay, Concentration Assay

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    Article Title: Rhinacanthus nasutus and okara polysaccharides attenuate colitis via inhibiting inflammation and modulating the gut microbiota.
    Article Snippet: Funding information Council of Agriculture, Grant/Award Number: 109AS-1.2.4-ST-a2 Abstract Plant polysaccharides have prebiotic properties for gut microbiota and immune modulation.. This study aimed to investigate the prevention abilities of edible Rhinacanthus nasutus polysaccharide (RNP) and okara polysaccharide (OP) in Sprague– Dawley rats with acetic acid-induced colitis.. The characterizations of RNP and OP were analyzed, including Fourier transform infrared, thermogravimetric analysis, differential scanning calorimetry, and monosaccharide composition.

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    Article Snippet: .. The sera were used for ELISA testing (Rat TNF‐α, Duo Set, Cat no: DY510‐05, R&D systems), (Rat INF‐γ, Duo Set, Cat no: RIF00, R&D systems), (Rat IL‐1β, Duo Set, Cat no: RLB00‐1, R&D systems), and (Rat IL‐6, Duo Set, Cat no: DY506‐05, R&D systems) for TNF‐α, INF‐γ, IL‐1β, and IL‐6 concentrations, respectively, as described by the manufacturer. ..

    Article Title: Acute Neuroinflammation Alters the Transport of a Model Therapeutic Protein from the Brain into Lymph and Blood.
    Article Snippet: The drainage of fluid and solutes along lymphatic pathways from the brain has been found to be impaired in mouse models of multiple sclerosis, Alzheimer’s disease, and Parkinson’s disease where neuroinflammation is present.. We recently demonstrated that 3H-albumin, a model therapeutic protein (∼65 kDa), undergoes preferential lymphatic transport from the brain using a cervical lymph cannulation model in healthy rats.. We thus hypothesized that neuroinflammation would impede the lymphatic transport of 3H-albumin from the brain.

    Article Title: Dysregulation of Amino Acid Transporters in a Rat Model of TLR7-Mediated Maternal Immune Activation.
    Article Snippet: .. Levels of IL-6 and IL-17a in rat maternal serum were determined using ELISA kits for rat IL-6 (#R6000B, sensitivity 36 pg/mL, R&D Systems, Minneapolis, MN, USA) and rat IL-17 (#NBP1-92705, sensitivity 1.0 pg/mL, Novus Biologicals, Centennial, CO, USA) according to manufacturer’s instructions. ..

    Article Title: Dysregulation of Amino Acid Transporters in a Rat Model of TLR7-Mediated Maternal Immune Activation
    Article Snippet: .. Levels of IL-6 and IL-17a in rat maternal serum were determined using ELISA kits for rat IL-6 (#R6000B, sensitivity 36 pg/mL, R&D Systems, Minneapolis, MN, USA) and rat IL-17 (#NBP1-92705, sensitivity 1.0 pg/mL, Novus Biologicals, Centennial, CO, USA) according to manufacturer’s instructions. ..

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    In Vitro:

    Article Title: Acute Neuroinflammation Alters the Transport of a Model Therapeutic Protein from the Brain into Lymph and Blood.
    Article Snippet: The drainage of fluid and solutes along lymphatic pathways from the brain has been found to be impaired in mouse models of multiple sclerosis, Alzheimer’s disease, and Parkinson’s disease where neuroinflammation is present.. We recently demonstrated that 3H-albumin, a model therapeutic protein (∼65 kDa), undergoes preferential lymphatic transport from the brain using a cervical lymph cannulation model in healthy rats.. We thus hypothesized that neuroinflammation would impede the lymphatic transport of 3H-albumin from the brain.

    Incubation:

    Article Title: An unbiased proteomics approach to identify the senescence-associated secretory phenotype of human bone marrow-derived mesenchymal stem cells.
    Article Snippet: .. Membranes were incubated overnight at 4 ◦C with specified dilutions of antibodies (ACTα2 [R&D Systems; MAB1420-SP], 1:1000; LTF [Abcam; ab135710], 1:500; SOD1 [Cell Signaling; 2770S],1:250; IL-6 [Cell Signaling; 12153], 1;1000; LTBP2 [R&D Systems; AF3850-SP], 1:1000; PXDN [Millipore; ABS1675], 1:500; OPG [R&D Systems; AF805-SP], 1:1000; SERPINE1 [Novus Biologicals; NBP1-19773], 1:1000; COL1α1 [Santa Cruz Biotechnology; sc-293182], 1:200; THBS1 [R&D Systems; AF3074], 1:200; against rat OPG [Santa Cruz Biotechnology; sc-390518], 1:1000; against rat IL-6 [R&D Systems; AF506], 1:200; against rat SOD1[Abcam; ab51254], 1:50,000) for target proteins. ..

    Article Title: An unbiased proteomics approach to identify the senescence-associated secretory phenotype of human bone marrow-derived mesenchymal stem cells
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    Inflammatory cytokine levels in the gastrocnemius muscle of rats in each group. ( a ) IL-1β; ( b ) IL-6; ( c ) TNF-α; ( d ) TGF-β (n = 4). Data are presented as mean ± SEM. Differences between groups were compared using one-way ANOVA. * p < 0.05, ns p > 0.05.

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    Figure Lengend Snippet: Inflammatory cytokine levels in the gastrocnemius muscle of rats in each group. ( a ) IL-1β; ( b ) IL-6; ( c ) TNF-α; ( d ) TGF-β (n = 4). Data are presented as mean ± SEM. Differences between groups were compared using one-way ANOVA. * p < 0.05, ns p > 0.05.

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    Techniques:

    (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐originated lymph vessels. (b) Effects of water intake on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake on changes in the concentration of IL‐6 in the lymph. (d) Effects of water intake on changes in the concentration of IL‐10 in the lymph. The open column, control; the black column, water intake. The error bars represent SDs.

    Journal: Physiological Reports

    Article Title: Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10

    doi: 10.14814/phy2.70891

    Figure Lengend Snippet: (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐originated lymph vessels. (b) Effects of water intake on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake on changes in the concentration of IL‐6 in the lymph. (d) Effects of water intake on changes in the concentration of IL‐10 in the lymph. The open column, control; the black column, water intake. The error bars represent SDs.

    Article Snippet: The concentrations of cytokines in the lymph were measured using enzyme‐linked immunosorbent assay (ELISA) kits: a rat IL‐1β ELISA quantitative kit (catalog no. RLB00; R&D Systems, Minneapolis, MN, USA), an IL‐6 ELISA kit (catalog no. R6000B; R&D Systems, Minneapolis, MN, USA), and a mouse/rat IL‐10 ELISA kit (catalog no. KE20003; Rosemont, IL, USA) (Arai et al., ).

    Techniques: Concentration Assay, Control

    (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐derived lymph vessels in the absence (white column) and presence of clodronate (oblique line column). (b) Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐1β in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐6 in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐10 in the lymph. The error bars represent SDs.

    Journal: Physiological Reports

    Article Title: Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10

    doi: 10.14814/phy2.70891

    Figure Lengend Snippet: (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐derived lymph vessels in the absence (white column) and presence of clodronate (oblique line column). (b) Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐1β in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐6 in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐10 in the lymph. The error bars represent SDs.

    Article Snippet: The concentrations of cytokines in the lymph were measured using enzyme‐linked immunosorbent assay (ELISA) kits: a rat IL‐1β ELISA quantitative kit (catalog no. RLB00; R&D Systems, Minneapolis, MN, USA), an IL‐6 ELISA kit (catalog no. R6000B; R&D Systems, Minneapolis, MN, USA), and a mouse/rat IL‐10 ELISA kit (catalog no. KE20003; Rosemont, IL, USA) (Arai et al., ).

    Techniques: Derivative Assay, Concentration Assay

    (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐derived lymph vessels in the absence (white column) and presence of a MyD88 inhibitor (oblique line column). (b) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐6 in the lymph. (d) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐10 in the lymph. The error bars represent the SDs.

    Journal: Physiological Reports

    Article Title: Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10

    doi: 10.14814/phy2.70891

    Figure Lengend Snippet: (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐derived lymph vessels in the absence (white column) and presence of a MyD88 inhibitor (oblique line column). (b) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐6 in the lymph. (d) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐10 in the lymph. The error bars represent the SDs.

    Article Snippet: The concentrations of cytokines in the lymph were measured using enzyme‐linked immunosorbent assay (ELISA) kits: a rat IL‐1β ELISA quantitative kit (catalog no. RLB00; R&D Systems, Minneapolis, MN, USA), an IL‐6 ELISA kit (catalog no. R6000B; R&D Systems, Minneapolis, MN, USA), and a mouse/rat IL‐10 ELISA kit (catalog no. KE20003; Rosemont, IL, USA) (Arai et al., ).

    Techniques: Derivative Assay, Concentration Assay